Part:BBa_K4103013:Design

ACLstart

This part is one of two parts that enable the construction of multiple transcriptional units using Golden Gate Assembly methods.

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal XbaI site found at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal XbaI site found at 10
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal XbaI site found at 10
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  

This part was designed by Scott Pownall as a part of the Open Yeast Collection - see https://stanford.freegenes.org/products/open-yeast-toolkit#bionet for more information

For the high-throughput screening of parts, it is always useful to be able to assemble multiple genomic constructs into one plasmid to enable quick and effective incorporation of your genes of interest as an episome (or into the genome of the target organism). Thus, we require flanking parts that enable each individual transcriptional unit to be assembled in the order of choice into a larger plasmid, which requires the use of matching, ordered restriction overhangs. Our overhang standard is based off the Open Yeast Collection standard developed for the production of protein in S. cerevisiae and Pichia pastoris See below for a helpful diagram about how to use this part (and functionally related parts from the OYC).

Design Notes

Source

https://stanford.freegenes.org/products/open-yeast-toolkit#bionet

===References===